Hydropuntia edulis as a nutritional therapeutic agent for human melanoma: In silico approach and in vitro validationfor functional food application

Enggar Yusrina Hasyyati; Maftuchah Rochmanti; Nafhah Hasan; Adha Fauzi Hendrawan; Arifa Mustika; Happy Kurnia Permatasari; Gioconda Millotti; Fahrul Nurkolis

Hydropuntia edulis as a nutritional therapeutic agent for human melanoma: In silico approach and in vitro validationfor functional food application

Enggar Yusrina Hasyyati ^[1] ; Maftuchah Rochmanti ^[1] ; Nafhah ^[1] ; Adha Fauzi Hendrawan ^[2] ; Arifa Mustika ^[1] ; Happy Kurnia Permatasari ^[3] ; Gioconda Millotti ^[4] ; Fahrul Nurkolis ^[1]
1. [1] Airlangga University
  
  Airlangga University
  
  Indonesia
2. [2] Gadjah Mada University
  
  Gadjah Mada University
  
  Indonesia
3. [3] University of Brawijaya
  
  University of Brawijaya
  
  Indonesia
4. [4] Juraj Dobrila University of Pula
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Localización: Nutrición clínica y dietética hospitalaria, ISSN 0211-6057, Vol. 45, Nº. 3, 2025, págs. 286-295
Idioma: inglés
DOI: 10.12873/453hasyyati
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Resumen
- Background: Melanoma is one of the deadliest forms ofskin cancer, with rising incidence and limited responsivenessto conventional chemotherapy. Marine macroalgae have re-cently emerged as a promising source of novel bioactive com-pounds with potential anticancer properties.
  
  Objective: Thisstudyaimedtoinvestigatetheanticar-cinogenicandantioxidantpotential of Hydropuntia edulis ex-tract (HEE) using integrated metabolomic profiling, in silicopredictions, and in vitro validation against melanoma.
  
  Methods: Untargeted LC-HRMS metabolomic profiling wasconducted to identify bioactive constituents in HEE. Key com-pounds were further analyzed through structure–activity rela-tionship (SAR), ADMET, and molecular docking simulations tar-geting melanoma-related proteins (BRAF, AKT1, EGFR, andTYRO3). Antioxidant and antiproliferative effects were assessedusing DPPH and MTT assays on B16-F10 melanoma cells.
  
  Results: Several metabolites including Sangivamycin,Michosterol C, Linamarin, and Elaiomycin K were identified.SAR analysis showed high antineoplastic probability forLinamarin (Pa = 0.831), Sangivamycin (Pa = 0.730), andMichosterol C (Pa = 0.759). Michosterol C showed strongbinding affinity to BRAF (−9.2 kcal/mol), comparable toDabrafenib (−9.1 kcal/mol). HEE demonstrated dose-depen-dent DPPH radical scavenging and inhibited melanoma cellproliferation with IC50 values similar to positive controls Conclusion: This study highlights Hydropuntia edulis as apromising source of nutraceutical agents against melanoma.Through a combination of metabolomic profiling, computationalprediction, and in vitro testing, HEE was shown to contain mul-tiple bioactive compounds with antioxidant and antiproliferativepotential. These findings suggest HEE could be further devel-oped as a dietary supplement or functional food to support nu-tritional management of melanoma patients, rather than as astand-alone chemotherapeutic. Future studies including com-pound isolation, in vivo validation, and clinical trials are neededto clarify its relevance, optimal dosing, and safety profile
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