Green algae Caulerpa racemosa inhibit proliferation and promote apoptosis in human HT-29 colon cancerby suppressing PI3K/AKT pathway

• Happy KURNIA PERMATASARI ^[1] ; Nurlinah AMALIA ^[1] ; Hikmawan WAHYU SULISTOMO ^[1] ; Kenty WANTRI ANITA ^[1] ; Husnul KHOTIMAH ^[1] ; Rudy KURNIAWAN ^[2] ; Fahrul NURKOLIS ^[3]
  1. [1] Brawijaya University, Malang, Indonesia
  2. [2] University of Indonesia–Cipto Mangunkusumo Hospital, Jakarta, Indonesia
  3. [3] State Islamic University of Sunan Kalijaga, Yogyakarta, Indonesia

  Mostrar afiliaciones +
• Localización: Nutrición clínica y dietética hospitalaria, ISSN 0211-6057, Vol. 44, Nº. 3, 2024, págs. 317-324
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Introduction: Colon cancer is a malignancy of the gastrointestinal which is one of the most common causes of death worldwide. Tumorigenesis involved oncologic pathway such asPI3K/AKT as the common pathway with the challenges is therapy resistance. Therefore, an alternative colon cancer treatments is needed that comes from biological resources such assea grapes.

    Objective: This study aims to determine anticancer potency of hexane extract from sea grapes (Caulerpa racemosa) on HT-29 cell line colon cancer based on suppress-ng PI3K/AKT pathway. Method: The study was conducted in vitro using the extract of Caulerpa racemosa at concentrations of (0, 400, 800,and 1.200) μg/mL on the HT-29 cell line based on the level expression of p-akt related to PI3K/AKT pathway, cleavedcaspase 3 related to apoptosis, and viable cells related to pro-liferation.

    Results: The research results was found that the hexaneextract of Caulerpa racemosa decreased the expression level of p-akt (Kruskal wallis, p=0,027), increased thecleaved caspase 3 (Kruskal wallis, p=0,016), and decreased he viable cells (Kruskal wallis, 24 and 48 hours: p=0,024and 0,034).

    Conclusion: Caulerpa racemosa hexane extract can inhibitcolon cancer cells growth by suppressing PI3K/AKT pathwaybased on inhibition proliferation and induce apoptosis.

• Referencias bibliográficas
  • Mattiuzzi C, Sanchis-Gomar F, Lippi G. Concise update on colorectal cancer epidemiology. Annals of translational medicine. 2019 Nov;7(21)....
  • Arnold M, Sierra MS, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global patterns and trends in colorectal cancer incidence and mortality....
  • Mattiuzzi C, Lippi G. Current cancer epidemiology. Journal of epidemiology and global health. 2019 Dec;9(4):217. doi: 10.2991/jegh.k.191008.001
  • Slattery ML, Mullany LE, Sakoda LC, Wolff RK, Stevens JR, Samowitz WS, Herrick JS. The PI3K/AKT signaling pathway: associations of miRNAs...
  • Danielsen SA, Eide PW, Nesbakken A, Guren T, Leithe E, Lothe RA. Portrait of the PI3K/AKT pathway in colorectal cancer. Biochimica et Biophysica...
  • Samsarga GW, Affandi Y, Utami NM, Nugraha IM, Manuaba IB. Negative Perceptions of Breast and Colorectal Cancer Patients Against Chemotherapy...
  • Marfuah I, Dewi EN, Rianingsih L. Study of the potential of sea grape extract (Caulerpa racemosa) as antibacterial against Escherichia coli...
  • Dissanayake IH, Bandaranayake U, Keerthirathna LR, Manawadu C, Silva RM, Mohamed B, Ali R, Peiris DC. Integration of in vitro and in-silico...
  • Manikandakrishnan M, Palanisamy S, Vinosha M, Kalanjiaraja B, Mohandoss S, Manikandan R, Tabarsa M, You S, Prabhu NM. Facile green route synthesis...
  • Mert-Ozupek N, Calibasi-Kocal G, Olgun N, Basbinar Y, Cavas L, Ellidokuz H. An Efficient and Quick Analytical Method for the Quantification...
  • Kerschbaum HH, Tasa BA, Schürz M, Oberascher K, Bresgen N. Trypan blue—Adapting a dye used for labelling dead cells to visualize pinocytosis...
  • Yang H, Liu JX, Shang HX, Lin S, Zhao JY, Lin JM. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways....
  • Wei R, Xiao Y, Song Y, Yuan H, Luo J, Xu W. FAT4 regulates the EMT and autophagy in colorectal cancer cells in part via the PI3K-AKT signaling...
  • Manandhar B, Kim HJ, Rhyu DY. Caulerpa okamurae extract attenuates inflammatory interaction, regulates glucose metabolism and increases insulin...
  • Pradhan B, Patra S, Nayak R, Behera C, Dash SR, Nayak S, Sahu BB, Bhutia SK, Jena M. Multifunctional role of fucoidan, sulfated polysaccharides...
  • Ferdous UT, Yusof ZN. Algal terpenoids: A potential source of antioxidants for cancer therapy. Terpenes and Terpenoids-Recent Advances. 2021...
  • Jiang M, Qi L, Li L, Li Y. The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer. Cell death discovery....
  • Li ZL, Mi J, Lu L, Luo Q, Liu X, Yan YM, Jin B, Cao YL, Zeng XX, Ran LW. The main anthocyanin monomer of Lycium ruthenicum Murray induces...