Comparison of The Effects of Low Flow Desfluran and High Flow Desfluran on Oxidative Stress and DNA Damage in Anesthesia Applications

• Hülya Tosun Söner ^[2] ; İbrahim Kaplan ^[1] ; Osman Uzundere ^[2] ; Serdar Söner ^[3] ; Feyzi Çelik ^[1]
  1. [1] Dicle University

     Dicle University

     Turquía

     
  2. [2] Department of Anesthesia, Health Science University Gazi Yaşargil Training and Research Hospital, Diyarbakır, Turkey
  3. [3] Department of Cardiology, Health Science University Gazi Yaşargil Training and Research Hospital, Diyarbakır, Turkey

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• Localización: Academic Journal of Health Sciences: Medicina Balear, ISSN-e 2255-0560, Vol. 39, Nº. 1, 2024, págs. 96-102
• Idioma: inglés
• DOI: 10.3306/AJHS.2024.39.01.96
• Títulos paralelos:
  • Comparación de los efectos del desflurano de bajo flujo y del desflurano de alto flujo sobre el estrés oxidativo y el daño del ADN en aplicaciones anestésicas
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• Resumen
  • español

    Antecedentes: Estudios han reportado que los anestésicos volátiles pueden afectar el estrés genotóxico y oxidativo en el presente estudio, nos propusimos comparar técnicas de anestesia de bajo y alto flujo en términos de daño al ADN y estrés oxidativo.

    Métodos: Se incluyeron 60 pacientes sometidos a anestesia con desflurano de alto y bajo flujo, y los sometidos a anestesia de bajo y alto flujo se clasificaron en los grupos I (n=31) y II (n=29), respectivamente. Se registraron los parámetros hemodinámicos y respiratorios de los pacientes y los valores de 8-OhdG (8-hidroxi desoxiguanosina ) para el daño del ADN (ácido desoxirribonucleico), MDA (Malondialdehído), cistatina C, TAS (estado antioxidante total), TOS (estado oxidativo total), y LPO (lıpıd peroxıd) valores de estrés oxidativo se midieron a partir de las muestras de sangre recogidas de los pacientes preoperatoriamente, intraoperatoriamente a la tercera hora y en el postoperatorio de veinticuatro horas.

    Resultados: El valor postoperatorio de MDA fue significativamente mayor en el grupo de alto flujo que en el de bajo flujo (p<0,05).

    No hubo diferencias significativas entre los grupos en cuanto a los valores de TAS, TOS, cistatina C, LPO y 8 OhdG (p>0,05).

    Conclusiones: En conclusión, los valores de MDA, un indicador de estrés oxidativo, aumentaron significativamente en la aplicación de desflurano a alto flujo. No hubo diferencias intergrupales en cuanto al daño del ADN.

  • English

    Background: Studies have reported that volatile anesthetics may affect genotoxic and oxidative stress in the present study, we aimed to compare low and high flow anesthesia techniques in terms of DNA damage and oxidative stress.

    Methods: We included 60 patients who underwent high and low flow desflurane anesthesia, and those who underwent low and high flow anesthesia were categorized in Groups I (n=31) and II (n=29), respectively. The hemodynamic and respiratory parameters of the patients were recorded and 8-OhdG (8-hydroxy deoxyguanosine) values for DNA (deoxyribonucleic acid) damage, MDA(Malondialdehyde), cystatin C, TAS (total antioxidant status), TOS (total oxidative status), and LPO (lıpıd peroxıd) values for oxidative stress were measured from the blood samples collected from the patients preoperatively, intraoperatively at the third hour and at the postoperative twenty-fourth hour.

    Results: Postoperative MDA value was significantly higher in the high flow group than in the low flow group (p<0.05). There was no significant intergroup difference in terms of TAS, TOS, cystatin C, LPO and 8 OhdG values (p>0.05).

    Conclusion: In conclusion, MDA values, an indicator for oxidative stress, increased significantly in high flow desflurane application.

    There was no intergroup difference in terms of DNA damage.

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