Stress is defined as the set of physiological reactions that prepare the organism for action. This research aims to determine the level of occupational stress in the health personnel of the Instituto Ecuatoriano de Seguridad Social (IESS) of Santo Domingo, 2024. The study sampled 94 nursing personnel, including the administrative and operational areas, adopting a quantitative approach, with a descriptive scope and a non- experimental cross-sectional design. It used the Nurse Stress Scale (NSS) as an instrument, which evaluates the physical, psychological and social state of the nursing staff. The results of the research determined that the nursing staff presents a medium level of work stress, represented by 65% of the participants; with a significant impact on the psychological dimension. In addition, it was possible to affirm that work overload, rotating schedules, insufficient remuneration and pressure from supervisors and colleagues are the main causes of this phenomenon. Similarly, it was found that working night shifts is related to greater emotional fatigue and high rates of health problems; significantly affecting the physical and emotional well-being of nurses, resulting in physical and psychological wear and tear of workers. In addition, it highlighted the importance of social support and implementing strategies to improve the working conditions and well-being of the nursing staff, in order to improve the quality of care and retain qualified personnel in the hospital.