Evaluación de la estabilidad genética de las cepas de referencia de Leptospira mantenidas bajo dos métodos de conservación

• Beltrán, Oscar Gabriel ^[1] ; Torres Higuera, Ligia Denise ^[1] ; Rodríguez Bautista, José Luis ^[2] ; Patiño Burbano, Rocio Esperanza ^[3]
  1. [1] Universidad Colegio Mayor de Cundinamarca

     Universidad Colegio Mayor de Cundinamarca

     Colombia

     
  2. [2] Universidade Federal Rural do Rio de Janeiro

     Universidade Federal Rural do Rio de Janeiro

     Brasil

     
  3. [3] Corporación colombiana de investigación agropecuaria-AGROSAVIA

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• Localización: NOVA, ISSN-e 2462-9448, ISSN 1794-2470, Vol. 20, Nº. 39, 2022, págs. 65-79
• Idioma: español
• DOI: 10.22490/24629448.6585
• Títulos paralelos:
  • Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods
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• Resumen
  • español

    Objetivo. Se evaluó la estabilidad genética de cepas de Leptospira spp., mantenidas bajo dos sistemas de conservación. Metodología. Se determinó el grado de conservación de los genes 16S rRNA y ompL1 de 10 serovares de referencia de la colección de Leptospira spp., pertenecientes al Sistema de Bancos de Germoplasma de la nación para la Alimentación y la Agricultura (SBGNAA). Resultados. Se corroboró que los genes evaluados estos no han sufrido cambios considerables, puesto que se evidenciaron similitudes superiores al 99,69% para 16S rRNA y de 99,02 % para ompL1, en los alineamientos pareados. Conclusión. Se comprobó la estabilidad genética y la pureza de las cepas de referencia de Leptospira spp., mantenidas en criopreservación en nitrógeno líquido a -196 °C y a temperatura ambiente durante ocho años aproximadamente.

  • English

    Objective. The genetic stability of Strains of Leptospira spp., maintained under two conservation systems, was evaluated. Methodology. The degree of conservation of the 16S rRNA and ompL1 genes of 10 reference serovars from the Leptospira spp. collection, belonging to the Sistema de Bancos de Germoplasma de la Nación para la Alimentación y la Agricultura (SBGNAA), was determined. Results. It was corroborated that the genes evaluated these have not undergone considerable changes, since similarities greater than 99.69 % were evidenced for 16S rRNA and 99.02% for ompL1, in the paired alignments. Conclusion. The genetic stability and purity of the reference strains of Leptospira spp. were verified. spp., kept in cryopreservation in liquid nitrogen at -196 °C and at room temperature for approximately eight years.

• Referencias bibliográficas
  • Prabhu N, Prabhu SS, Thanveer M, Revathi N, Josna N, Darsana R, et al. Differentiation of parasitic and saprophytic leptospirae by oxidase...
  • Wuthiekanun V, Amornchai P, Paris DH, Langla S, Thaipadunpanit J, Chierakul W, et al. Rapid Isolation and Susceptibility Testing of Leptospira...
  • Ferreira AS, Costa P, Rocha T, Amaro A, Vieira ML, Ahmed A, et al. Direct detection and differentiation of pathogenic Leptospira species using...
  • Cerqueira GM, Picardeau M. A century of Leptospira strain typing. Infect Genet Evol. 2009;9(5):760–8.
  • Balamurugan V, Gangadhar NL, Mohandoss N, Thirumalesh SRA, Dhar M, Shome R, et al. Characterization of leptospira isolates from animals and...
  • Mgode GF, Machang RS, Collares-pereira M, Vieira ML, Goris MG a, Engelbert M, et al. Challenges in determining the pathogenicity status of...
  • Chang YF, Chen CS, Palaniappan RUM, He H, McDonough SP, Barr SC, et al. Immunogenicity of the recombinant leptospiral putative outer membrane...
  • Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Bailey MS, Holden MTG, et al. A Single Multilocus Sequence Typing (MLST) Scheme...
  • Boonsilp S, Thaipadungpanit J, Amornchai P, Wuthiekanun V, Chierakul W, Limmathurotsakul D, et al. Molecular detection and speciation of pathogenic...
  • Vincent AT, Schiettekatte O, Goarant C, Neela VK, Bernet E, Thibeaux R, et al. Revisiting the taxonomy and evolution of pathogenicity of the...
  • Masuzawa T, Saito M, Nakao R, Nikaido Y, Matsumoto M, Ogawa M, et al. Molecular and phenotypic characterization of Leptospira johnsonii sp....
  • Caimi K, Ruybal P. Leptospira spp., a genus in the stage of diversity and genomic data expansion. Infect Genet Evol. 2020;81(August 2019).
  • Cilia, Giovanni; Bertelloni F, Albini S, Fratini F. Insight into the Epidemiology of Leptospirosis : A Review of. Animals. 2021;11(191):1–16.
  • Guglielmini J, Bourhy P, Schiettekatte O, Zinini F, Brisse S, Picardeau M. Genus-wide Leptospira core genome multilocus sequence typing for...
  • Koval AA, Brihuega BF, Grune Loffler S, López S, Saint Martin M, Lagioia GG, et al. First isolation of Leptospira borgpetersenii serovar Hardjo...
  • Miller E, Barragan V, Chiriboga J, Weddell C, Luna L, Jiménez DJ, et al. Leptospira in river and soil in a highly endemic area of Ecuador....
  • Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, et al. Genome reduction in Leptospira borgpetersenii reflects limited transmission...
  • Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, et al. Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome...
  • Picardeau M, Bulach DM, Bouchier C, Zuerner RL, Zidane N, Wilson PJ, et al. Genome sequence of the saprophyte Leptospira biflexa provides...
  • Nascimento A, Ko A, Martins E, Monteiro B, Ho P, Haake D, et al. Comparative Genomics of Two Leptospira interrogans Serovars Reveals Novel...
  • Haake DA, Chao G, Zuerner RL, Barnett JK, Barnett D, Mazel M, et al. The leptospiral major outer membrane protein LipL32 is a lipoprotein...
  • Philip N, Garba B, Neela VK. Long-term preservation of Leptospira spp.: challenges and prospects. Appl Microbiol Biotechnol. 2018;102(13):5427–35.
  • Cerqueira GM, McBride AJ., Queiroz A, Pinto LS, Silva ÉF, Hartskeerl RA, et al. Monitoring Leptospira strain collections: The need for quality...
  • Borrero R, Batista N, Blain K, Valdés Y. Conservación de cepas vacunales de Leptospira en nitrógeno líquido. Rev Cubana Med Trop. 2010;62(2):130–7.
  • Borrero R, González A, del Puerto C, Batista N, Valdés Y. Conservación de cepas vacunales de Leptospira a -70 oC. Rev Cubana Med Trop. 2006;58(1):50–5.
  • Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for...
  • Morey RE, Galloway RL, Bragg SL, Steigerwalt AG, Mayer LW, Levett PN. Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing....
  • Cerqueira GM, McBride AJA, Hartskeerl RA, Ahmed N, Dellagostin OA, Eslabão MR, et al. Bioinformatics describes novel loci for high resolution...
  • Haake DA, Suchard MA, Kelley MM, Dundoo M, Alt DP, Zuerner RL. Molecular evolution and mosaicism of leptospiral outer membrane proteins involves...
  • Rajapakse S, Rodrigo C, Handunnetti SM, Fernando DD. Current immunological and molecular tools for leptospirosis: Diagnostics, vaccine design,...
  • Feng CY, Li QT, Zhang XY, Dong K, Hu BY, Guo XK. Immune strategies using single-component LipL32 and multi-component recombinant LipL32-41-OmpL1...
  • Lin X, Sun A, Ruan P, Zhang Z, Yan J. Characterization of conserved combined T and B cell epitopes in leptospira interrogans major outer membrane...
  • OIE. Leptospirosis. In: Terrestrial Manual. 2018. p. 503–16.
  • Fearnley C, Wakeley PR, Gallego-Beltran J, Dalley C, Williamson S, Gaudie C, et al. The development of a realtime PCR to detect pathogenic...
  • Suzuki MT, Giovannoni SJ. Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Microbiol....
  • Rosario LA, Arencibia DF, Batista N, Jirón W, Suárez YE, Infante JF. Caracterización de aislamientos clínicos de leptospira por métodos fenotípicos...
  • Braun RC, Pedretti KT, Casavant TL, Scheetz TE, Birkett CL, Roberts CA. Parallelization of local BLAST service on workstation clusters. Futur...
  • Thompson J, Higgins D, Gibson T. ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,...
  • Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870–4.
  • Agudelo-Flórez P, Londoño AF, Quiroz VH, Ángel JC, Moreno N, Loaiza ET, et al. Prevalence of Leptospira spp. in urban rodents from a Groceries...
  • Dong H, Hu Y, Xue F, Sun D, Ojcius DM, Mao Y, et al. Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity...
  • Dezhbord M, Esmaelizad M, Khaki P, Fotohi F, Moghaddam AZ. Molecular identification of the ompL1 gene within Leptospira interrogans standard...
  • Reitstetter RE. Development of species-specific PCR primer sets for the detection of Leptospira. FEMS Microbiol Lett. 2006;264(1):31–9.
  • Fernandes LG V., Vieira ML, Kirchgatter K, Alves IJ, de Morais ZM, Vasconcellos SA, et al. OmpL1 Is an Extracellular Matrix- and Plasminogen-Interacting...
  • Vedhagiri K, Natarajaseenivasan K, Chellapandi P, Prabhakaran SG, Selvin J, Sharma S, et al. Evolutionary Implication of Outer Membrane Lipoprotein-Encoding...
  • Higuera LDT, Ortega DO, Bautista JLR, Burbano REP. Comparación de tres tratamientos para la crioconservación de serovares de Leptospira en...
  • Wangroongsarb P, Chanket T, Gunlabun K, Long DH, Satheanmethakul P, Jetanadee S, et al. Molecular typing of Leptospira spp. based on putative...
  • Romero EC, Blanco RM, Galloway RL. Analysis of multilocus sequence typing for identification of Leptospira isolates in Brazil. J Clin Microbiol....
  • Bourhy P, Collet L, Lernout T, Zinini F, Hartskeerl RA, Van Der Linden H, et al. Human Leptospira isolates circulating in Mayotte (Indian...
  • Verma A, Soto E, Illanes O, Ghosh S, Fuentealba C. Detection and genotyping of Leptospira spp. From the kidneys of a seemingly healthy pig...
  • Rettinger A, Krupka I, Grünwald K, Dyachenko V, Fingerle V, Konrad R, et al. Leptospira spp. strain identification by MALDI TOF MS is an equivalent...
  • Tian RM, Cai L, Zhang WP, Cao HL, Qian PY. Rare events of intragenusand intraspecies horizontal transfer of the 16S rRNA gene. Genome Biol...
  • Tiley GP, Poelstra JW, dos Reis M, Yang Z, Yoder AD. Molecular Clocks without Rocks: New Solutions for Old Problems. Trends Genet. 2020;36(11):845–56.
  • Kuo CH, Ochman H. Inferring clocks when lacking rocks: The variable rates of molecular evolution in bacteria. Biol Direct. 2009;4(September...
  • Bierque E, Thibeaux R, Girault D, Soupé-Gilbert ME, Goarant C. A systematic review of Leptospira in water and soil environments. PLoS One....
  • Cooper VS, Vohr SH, Wrocklage SC, Hatcher PJ. Why genes evolve faster on secondary chromosomes in bacteria. PLoS Comput Biol. 2010;6(4).