Lymphotherapy induce an increase of blocking factors and correct infertility problems

• Manrique, Edwin ^[1] ; Rincón, Verónica ^[1] ; Ossa, Humberto ^[2]
  1. [1] Universidad Distrital Francisco José de Caldas

     Universidad Distrital Francisco José de Caldas

     Colombia

     
  2. [2] Laboratory of Genetic and Molecular Biology. Bogotá, D.C. Colombia.
• Localización: NOVA, ISSN-e 2462-9448, ISSN 1794-2470, Vol. 11, Nº. 19, 2013, págs. 25-31
• Idioma: español
• DOI: 10.22490/24629448.1020
• Títulos paralelos:
  • Lymphotherapy induce an increase of blocking factors and correct infertility problems
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• Resumen
  • español

    This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique. It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

  • English

    This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique.It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

• Referencias bibliográficas
  • Beer A, Billingham R.E. The embryo as a transplant. In: A Scientific American. 1974; 230: 36 - 46.
  • Maldonado J. Efecto de la aloinmunoterapia en la reproducción animal. Tesis de grado como requisito parcial para optar al título de Magíster...
  • Ossa J, Sánchez F, Cadavid A. Inmunología de la Reproducción. Primera Edición. Imprenta Universidad de Antioquía. Colombia. 1993. 206 p.
  • Ossa H, Benítez A, Cárdenas S, Moreno C.H. Cuantificación de factores bloqueadores del cultivo mixto de linfocitos en mujeres abortadoras...
  • Medicina Reproductive. 2000; 3(1): 33 - 36
  • Gurka G., Rocklin R.E. Reproductive Immunology. In: JAMA.1987;258 (20): 2983 – 7.
  • Lancet. Maternal blocking antibodies, the fetal allograft and recurrent abortion. The Lancet. 1983.
  • Mossmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and citotoxicity assays. In: Journal of...
  • PROMEGA. 1994. CELL TITER 96 TM Aqueous Non – Radioactive cell proliferation Assay. Technical Bulletin No 169.
  • PROMEGA. 1995. CELL TITER 96 TM Non – Radioactive cell proliferation Assay. Technical Bulletin No 112.
  • Daniel W. Bioestadística. Base para el análisis de ciencias de la salud. Editorial Uteha. Noriega editores. México. 1997; 878p.
  • Reyes C. 1995. Bioestadística Aplicada. Agronomía, Biología, Química. Editorial Trillas. México. 216p.
  • Mowbray J, Gibbings C; Liddell H; Reginald P; Underwood J; Beard R. Controlled trial of treatment of recurrent spontaneous abortion by immunisation...
  • Ober C, Karrison T, Odem R; Barnes R, Branch W; Stephenson M, Baron B, Ann M, Scott J, Schreiber J. Mononuclear cell immunisation in prevention...
  • Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The amount and distribution of loss before and after implantation....
  • Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The effect of transferring varying numbers of eggs. In: Journal...
  • Adams C. E. Maintenance of pregnancy relative to the presence of few embryons in the rabbit. In: Journal of Endocrin. 1970; 48: 243 - 249.
  • Benitez A., Cardenas. Determinación de factores bloqueadores mediante el cultivo mixto de linfocitos (CML), técnica MTT. Tesis de pregrado....
  • Scott Jr, Park M, Edwin S, Brach D. Interpretation of blocking activity in maternal serum depends on the equation used for calculation of...