Modelo Animal de Anemia Inducida por Flebotomía Crónica: Relación Funcional Entre Hierro y Eritropoyesis

• Castillo Bohórquez MSC, Martha ^[1] ; D´Anna, María ^[2] ; Gaona Prieto, Suani ^[1] ; García, Betina ^[2] ; Giorgi, Gisela ^[2] ; Mora Bautista MSC, Ana Isabel ^[1] ; Roque, Marta ^[2] ; Villarraga Muñoz, María del Mar ^[1]
  1. [1] Universidad Colegio Mayor de Cundinamarca

     Universidad Colegio Mayor de Cundinamarca

     Colombia

     
  2. [2] Universidad Nacional del Sur

     Universidad Nacional del Sur

     Argentina

     
• Localización: NOVA, ISSN-e 2462-9448, ISSN 1794-2470, Vol. 10, Nº. 17, 2012, págs. 50-63
• Idioma: español
• DOI: 10.22490/24629448.517
• Enlaces
  • Texto completo
• Dialnet Métricas: 3 Citas
• Resumen
  • español

    En el presente estudio se analizo la respuesta eritropoyética a la anemia inducida por flebotomía crónica y los cambios en la distribución del hierro celular y sistémico del organismo. Ratones hembra de la cepa CF1 (n=32), se dividieron en dos lotes: control y experimental, siguiendo un diseño experimental pareado según su peso. La distribución del hierro en bazo e hígado durante la flebotomía crónica fue evaluada mediante estudios morfológicos y la actividad eritropoyética mediante estudios hematológicos. Las diferencias estadísticas se determinaron mediante Test Student. El nivel de significancia se fijó en p

  • English

    In the present work we studied the erythropoietic response to chronic phlebotomy-induced anemia and changes in cellular and systemic body iron distribution. Female mice (CF1 strain, n =32) were divided into control and experimental groups, following a paired experimental design. Iron distribution in spleen and liver during chronic phlebotomy was assessed by morphological studies and erythropoietic activity by hematological studies. Statistical differences were determined by Student test. The significance level was set at p

• Referencias bibliográficas
  • Pérez G, Vittori D, Pregi N, Garbossa G, Nesse A. Homeostasis del hierro. Mecanismos de absorción, captación celular y regulación. Acta Bioquím...
  • Dunn L, Suryo Y, Richardson D. Iron uptake and metabolism in the new millennium. Trends Cell Biol 2006; 17 (2): 93-100.
  • Ganz T. Nement E. Regulation of iron acquisition and iron distribution in mammals. Acta Biochim Biophys. 2006; 1763: 690- 9.
  • Shayeghi M, Latunde-Dada GO, Oakhill JS, Laftah AH, Takeuchi K, Halliday N, et al. Identification of an intestinal heme transporter. Cell...
  • Latunde-Dada GO, Westhuizen JV, Vulpe CD, Anderson GJ, Simpson RJ, McKie AT. Molecular and functional roles of duodenal cytochrome B (Dcytb)...
  • Mackenzie B, Garrick MD. Iron imports. II. Iron uptake at the apical membrane in the intestine. Am J Physiol Gastrointest Liver Physiol 2005;...
  • Torti FM, Torti S. Regulation of ferritin genes and protein. Blood 2002; 99: 3505-16.
  • Matsuno T, Mori M, Awai M. Distribution of ferritin and hemosiderin in the liver, spleen and bone marrow of normal, phlebotomized and iron...
  • D’Anna C, Veuthey T, Roque M. Immunolocalization of Ferroportin in Healthy and Anemic Mice. J Histochem Cytochem. 2009; 57(1): 9– 16.
  • Abboud S, Haile DJ. A novel mammalian iron-regulated protein involved in intracellular iron metabolism. J Biol Chem 2000; 275: 19906-12.
  • Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, et al. Hepcidin regulates cellular iron efflux by binding to ferroportin and...
  • Ganz T, Brissot P, Cohen A. Hepcidin and Its Role in Regulating Systemic Iron Metabolism. Am Soc Hematol. 2006; 1: 29- 35.
  • Muckenthaler M, Galy B, Hentze M. Systemic iron homeostasis and the Ironresponsive element/iron regulatory protein (IRE/IRP) regulatory network....
  • Viatte L, Vaulont S. Hepcidin, the iron watcher. Biochimie 2009; 1-6.
  • Pantapoulos K. Function of the hemochromatosis protein HFE: lessons from animal models. World J Gastroenterol 2008; 14(45): 6893-901
  • Babitt JL, Huang FW, Xia Y, Sidis Y, Andrews NC, Lin HY. Modulation of bone morphogenetic protein signaling in vivo regulates systemic iron...
  • Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D. Liver iron transport. World J Gastroenterol 2007; 13: 4725-36.
  • Tabuchi M, Yanatori I, Kawai Y, Kishi F. Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron...
  • Knutson MD, Wessling-Resnick M. Iron metabolism in the reticuloendothelial system. Crit Rev Biochem Mol 2003: 38: 61-88.
  • Shaw J, Friedman J. Iron Deficiency Anemia: Focus on Infectious Diseases in Lesser Developed Countries. Anemia. 2011; 1- 10.
  • Roque M, Gatti C, Aggio M. Estudios para evaluar el hierro corporal. Ars Pharm. 2005; 46 (2): 181- 91.
  • Arribas J, Vallina E. Hematología Clínica Temas de Patología Médica Oviedo. Textos universitarios ediuno. 2005. p 55-8.
  • Kellera G, Lacauda G, Robertsona S. Development of the hematopoietic system in the mouse. Rev Clin Exp Hematol. 1999; 27: 777– 87.
  • Guide for the care and use of laboratory animals. National Research Council. Washington DC, 1996.
  • Fox J, Barthold S, Davisson M, Newcomer C, Quimby F, Smith F. The Mouse in Biomedical Research: Diseases. Ed. ELSEVIER. 2007; p142-50.
  • Roque M, D´Anna C, Gatti C, Veuthey T. Hematological and Morphological Analysis of the Erythropoietic Regenerative Response in Phenylhydrazine-induced...
  • D´Anna C, Gatti C, Veuthey T, Sánchez M, Roque M. Eritropoyesis y Esplenectomía en un Modelo Murino. Rev Cientf AMBB. 2006; 16 (4): 88- 9
  • Camberlein E, Abgueguen E, Fatih N, François Hergaux C, Leroyer P, Turlin B, et al. Hepcidin induction limits mobilisation of splenic iron...
  • Latunde-dada GO, AT McKie & RJ Simpson: Animal models with enhanced erythropoiesis and iron absorption. Biochim Biophys Acta 2006, 1762,...