Células Dendríticas (CDs) diferenciadas a partir de Monocitos humanos como herramienta para el estudio de agentes antileishmaniales

• Delgado, Gabriela ^[2] ; Granados, Diana ^[1]
  1. [1] Universidad Nacional de Colombia

     Universidad Nacional de Colombia

     Colombia

     
  2. [2] Grupo de Investigación en Inmunotoxicología, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia. Programa Doctoral en Biociencias, Facultad de Ingeniería, Universidad de la Sabana.
• Localización: NOVA, ISSN-e 2462-9448, ISSN 1794-2470, Vol. 6, Nº. 10, 2008, págs. 162-169
• Idioma: español
• DOI: 10.22490/24629448.407
• Títulos paralelos:
  • Dendritic Cells From Human Monocytes: As a Tool to Study Antileishmanials Agents
• Enlaces
  • Texto completo
• Resumen
  • español

    Las células dendríticas son consideradas potentes células presentadoras de antígeno, fundamentales para el inicio y desarrollo de una respuesta inmunitaria efectiva frente a diversos agentes infecciosos. El estudio de la interacción parásito-células dendríticas, es esencial para la evaluación y desarrollo de nuevas estrategias terapéuticas profilácticas capaces de controlar de una manera segura y efectiva el agente patógeno, si se tiene en cuenta que diferentes agentes patógenos (ej: parásitos perteneciente al género Leishmania) pueden afectar la maduración y función de las células dendríticas, como uno de los principales mecanismos de evasión de la respuesta.En el presente estudio, nosotros determinamos que las células dendríticas derivadas de monocitos de sangre periférica humana diferenciadas luego de 72 horas de cultivo en presencia de interleuquina 4 y factor estimulador de colonias granulocito/macrófago, pueden ser utilizadas exitosamente para la evaluación de la actividad anti-leishmanial e inmuno-moduladora de nuevas formulaciones terapéuticas para el control de la leishmaniosis.

  • English

    Dendritic cells, considered as powerful antigen presenting cells, are fundamental fir the beginning and development of an effective immune response against diverse infections agents. The study of interaction between dendritic cells and parasites is essential for the evaluation and development of new prophylactic and therapeutics strategies able to control the pathogenic agent in a safe and effective way. This is important when considering that different pathogenic agents (e.g. Leishmania parasites) can affect the maturation process and dendritic cells function, as an effective mechanism to evade the immune response.In this study, it was determined that dendritic cells differentiated from human peripheral blood monocytes (after 72 hours culture in presence of 4 interleukin and granulocyte/macrophage colonies stimulating factor) can successfully be used for evaluation of anti-leishmanial activity and immunomodulator effect of new therapeutics formulations for leishmaniasis control.

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