Bodas de plata de la reacción en cadena de la polimerasa (PCR)

• Cubillos, Karen ^[2] ; Pinilla MSC, Gladys ^[1] ; Rodríguez, Monica ^[3]
  1. [1] Universidad Colegio Mayor de Cundinamarca

     Universidad Colegio Mayor de Cundinamarca

     Colombia

     
  2. [2] Instituto de Biotecnología Universidad Nacional, Bogotá - Colombia.
  3. [3] Universidad Nacional Posgrado de Microbiología

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• Localización: NOVA, ISSN-e 2462-9448, ISSN 1794-2470, Vol. 6, Nº. 9, 2008, págs. 65-75
• Idioma: español
• DOI: 10.22490/24629448.397
• Títulos paralelos:
  • Silver Anniversary of the Polymerase Chain Reaction (Pcr)
• Enlaces
  • Texto completo
• Dialnet Métricas: 2 Citas
• Resumen
  • español

    Invenciones verdaderamente revolucionarias han promovido el cambio de pensamiento y la manera de trabajar en el ámbito del laboratorio. Una de estas invenciones es la reacción en cadena de la polimerasa, la cual ha aportado de manera significativa al conocimiento científico. Las diferentes metodologías que aplican la reacción en cadena de la polimerasa han permitido a los investigadores manipular la información genética de los organismos, facilitando procedimientos como la clonación y la secuenciación, entre otros, lo cual agilizó significativamente los resultados del Proyecto Genoma Humano. Existe diversidad de variantes de la reacción en cadena de la polimerasa convencional. Este escrito tiene por objetivo presentar una revisión sobre el tema, especialmente sobre la reacción en cadena de la polimerasa en tiempo real, debido a las ventajas que ofrece.

  • English

    Truly revolutionary inventions have promoted the change of thought and the way to work in the laboratory. One of these inventions is the polymerase chain reaction (PCR), which has contributed significantly to the scientific knowledge. The different methodologies that use the polymerase chain reaction have allowed investigators to manipulate the genetic information of organisms, facilitating procedures like cloning and sequencing, among others, which sped the Human Genome Project results significantly. There are several variants of the conventional polymerase chain reaction. This work tries to present a revision on the subject, especially on the Real-time polymerase chain reaction, due to the advantages that it offers.

• Referencias bibliográficas
  • Mullis BK. The unusual origin of the Polymerase Chain Reaction. Sci Am. 1990;262:56-65.
  • McPherson JD, Marra M, Hillier L, Waterston RH, Chinwalla A, Wallis J, et al. A physical map of the human genome. Nature. 200;409:934-941.
  • Rodriguez IP, Barrera H. La reaccion en cadena de la polimerasa a dos decadas de su invencion. Ciencia UANL.2004;7:323-332.
  • Mocellin S, Rossi CR, Pilati P, Nitti D,Marincola FM. Quantitative real-time PCR: a powerful ally in cancer research. Trends Mol Med. 2003;9:189-195.
  • Templeton KE, Scheltinga SA, Graffelman WA, Van Schie JM, Crielaard JW, Sillekens P, et al. Comparison and evaluation of realtime PCR, real-time...
  • Costa J. Reaccion en cadena de la polimerasa (PCR) a tiempo real. Enferm Infecc Microbiol Clin. 2004;22:299-305.
  • Luque JC, Herraez SA. Texto ilustrado de Biologia Molecular e Ingenieria Genetica. Ed. Harcourt.2000. pg 313.
  • Innia M, Gelfand D, Sninsky J. PCR strategies. Academic Press New York USA.1995:325.
  • Choi S, Kim S-H, Kim H-J, Lee D-G, Choi J-H, Yoo J-H, et al. Multiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes...
  • Jimenez A, Gomez R, Agirre X, Barrios M, Navarro G, Eneriz E, et al. PCR en tiempo real, una nueva herramienta para la toma de decisiones...
  • Kaltenboeck B, Wang C. Advances in real-time PCR: application to clinical laboratory diagnostics. Adv Clin Chem. 2005;40:219-259.
  • Heid CA, Stevens J, Livak JK, Williams M. Real Time Quantitative. Genome Res.1996;6:986-994.
  • Equipos para RT-PCR. Disponible en www.Biorad.com.
  • Rutledge RG, Cote C. Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res. 2003;31:3-6.
  • Bustin SA, Mueller R. Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin Sci.2005;109:365–379.
  • Kubista M, Andrade JM, Bengtsson M, Forootan A, Jonak J, Lind K, et al. The real-time polymerase chain reaction. Mol Aspects Med. 2006;27:95–125.
  • Wong M, Medrano JF. Real-time PCR for mRNA quantitation. Biotechniques. 2005;39:75-85.
  • Peirson S, Butler J, Foster R. Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic...
  • Pfaffl M. A new mathematical model for relative quantification in Real-Time RT-PCR. Nucleic Acids Res. 2001;29:e45.
  • Kainz P. The PCR plateau phase - towards an understanding of its limitations. Biochim Biophys Acta. 2000;1494:23-27.
  • Al-Soud WA, Jhonsson LJ, Radstrom P. Identification and Characterization of Immunoglobulin G in Blood as a Major Inhibitor of Diagnostic PCR....
  • Al-Soud WA, Radstrom P. Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol. 2001;39:485-493.
  • Gudnason H, Dufva M, Bang DD, Wolff A. Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition...
  • Nazarenko I, Lowe B, Darfler M, Ikonomi P, Schuster D, Rashtchian A. Multiplex quantitative PCR using self-quenched primers labeled with a...
  • Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol. 2002;29:23–39.
  • Provenzano M, Rossi C, Mocellin S. The usefulness of quantitative real-time PCR in immunogenetics. Third Quarter. 2001:89-91.
  • Burggraf S, Olgemoller B. Simple technique for internal control of real-time amplification assays. Clin Chem. 2004;50:819–825.
  • Memorias Curso teorico-practico de PCR en tiempo real. Corpogen. Julio 26 y 27 de 2007.
  • Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, et al. Real-time PCR in clinical microbiology:applications for routine laboratory...
  • Yuan J, Reed A, Chen F, Stewart N. Statistical analysis of real-time PCR data. BMC Bioinformatics. 2006;85:1471-2105.
  • Pfaffl M, Hageleit M. Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time...
  • Gibson U, Heid CA, Williams PM. A Novel method for real time quantitative RT-PCR. Genome Res.1996;6:995-1001.
  • Livak K, Schmittgen T. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods....
  • Zucol F, Ammann RA, Berger C, Aebi C, Altwegg M, Niggli FK, et al. Real-time quantitative broad range PCR assay for detection of the 16S rRNA...
  • Vandecasteele SJ, Peetermans WE, Merckx R, Eldere V. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman...
  • Rodicio M, Mendoza M. Identificacion bacteriana mediante secuenciacion del ARNr 16S:fundamento,metodologia y aplicaciones en microbiologia...
  • Beck B, Hoelsmeyer M, Paul S, Downs D. A mutation in the essential gene gmk (encoding guanlyate kinase) generates a requirement for adenine...
  • Skurray R, Brow M. Identification of Suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance systems...
  • Unkles S, Logsdon J, Robinson K, Kinghorn J, Duncan J. The tpiA gene is a transcripcional isomerase and glyceraldehyde-3-phosphate dehydrogenase...
  • Xu Y, Hong Y, Hall T. Rice triosephosphate isomerase gene 5’ sequence directs β-glucuronidase activity in transgenic tobacco but requires...
  • Plamannt D. Michael, Stauffer VG. Regulation of the Escherichia coli glyA Gene by the metR Gene Product and Homocysteine. J Bacteriol. 1989;171:4958-4962.
  • Purvis IJ, Loughlin L, Bettany AJ, Brown AJ. Translation and stability of an Escherichia coli beta galactosidase mRNA expressed under the...
  • Poysti J, Nathan, Oresnik J. Characterization of Sinorhizobium meliloti triose phosphate isomerase genes. J Bacteriol. 2007;189:3445–3451.
  • Schmittgen TD, Zakrajsek BA. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR....
  • Rubie C, Kempf K, Hans J, Su T, Tilton B, Georg T, et al. Housekeeping gene variability in normal and cancerous colorectal, pancreatic, esophageal,...
  • Dheda K, Huggett J, Bustin S, Johnson M, Rook G, Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR....
  • Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005;6:279–284.
  • Bonilla E, Parraga M, Lopez LA, Escolar F, Del Marzo J. Cuantificacion de la expresion genica a partir de un numero limitado de celulas mediante...
  • Burkhard M, Charlotta L, Wagner M, Kra¨mer N, Hoorfar J. Enumeration of Salmonella bacteria in food and feed samples by real-time pcr for...
  • De Medici D, Croci L, Delibato E, Di Pasquale S, Filetici E, Toti L. Evaluation of DNA extraction methods for use in combination with SYBR...
  • Burkhard M, Paccassoni E, Fach P, Bunge C, Martin A, Helmuth R. Diagnostic Real-Time PCR for detection of Salmonella in food. Appl Environ...
  • Artin I, Bjorkman P, Cronqvist J, Radstrom P, Holst E. First case of type E wound botulism diagnosed using real-time PCR. J Clin Microbiol....
  • Fenicia L, Anniballi F, De Medici D, Delibato E, Aureli P. SYBR green real-time PCR method to detect Clostridium botulinum type A. Appl Environ...
  • Yoon SY, Chung GT, Kang DH, Ryu C, Yoo CK, Seong WK. Application of real-time pcr for quantitative detection of Clostridium botulinum Type...
  • Edwards KJ, Kaufmann ME, Unders NA. Rapid and accurate identification of coagulase-negative staphylococci by real-time PCR.. J Clin Microbiol....
  • Kane TD, Alexander JW, Johannigman JA. The detection of microbial DNA in the blood: a sensitive method for diagnosing bacteremia and/or bacterial...
  • Cursons RT, Jeyerajah E, Sleigh JW. The use of polymerase chain reaction to detect septicemia in critically ill patients. Crit Care Med.1999;27:937–940.
  • Lu JJ, Perng CL, Lee SY, Wan CC. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification...
  • Rantakokko-Jalava K, Nikkari S, Jalava J, Eerola E, Skurnik M, Meurman O, et al. Direct amplification of rRNA genes in diagnosis of bacterial...
  • Mariani BD, Martin DS, Levine MJ, Booth RE Jr, Tuan RS. The coventry award polymerase chain reaction detection of bacterial infection in total...
  • Klaschik S, Lehmann LE, Raadts A, Book M, Hoeft A, Stuber F. Real-time PCR for detection and differentiation of gram-positive and gram-negative...
  • Rosato EA, Craig A, William, Archer LG. Quantitation of mecA transcription in oxacillin-resistant Staphylococcus aureus clinical isolates....
  • Volkmann H, Schwartz T, Bischoff P, Kirchen S, Obst U. Detection of clinically relevant antibiotic resistance genes in municipal wastewater...
  • Theis T, Skurray AR, Brown HM. Identification of suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance...
  • Diederen B, Kluytmans J, Vandenbroucke CM, Peeters MF. Utility of Real-Time PCR for Diagnosis of Legionnaires’ Disease in Routine Clinical...
  • Stolhaug A, Bergh K. Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S...
  • Templeton KE, Scheltinga SA, Graffelman AW, Van Schie JM, Crielaard JW, Sillekens P, et al. Comparison and evaluation of real-time PCR, real...
  • Michelow IC, Olsen K, Lozano J, Rollins NK, Duffy LB, Ziegler T, et al. Epidemiology and clinical characteristics of communityacquired pneumonia...
  • Gullsby K, Storm M, Bondeson K. Simultaneous Detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by Use of Molecular Beacons in...
  • Welti, Jaton MK, Altwegg M, Sahli R, Wenger A, Bille J. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae,...