Acceso usuarios
Judith Figueroa, Patricia Martínez Lanz, Zulma Johanna Moreno Huertas
Se evaluó la actividad antimicrobiana de cuatro extractos de propóleos argentinos, cinco colombianos y uno cubano frente a Streptococcus mutans ATCC 25175. La actividad bactericida y bacteriostática fue medida por concentración mínima inhibitoria en un rango entre 0.02 y 15 mg/ml. La totalidad de las muestras analizadas presentaron actividad contra Streptococcus mutans a concentraciones de 15 a 3.75 mg/ml. Los propóleos que presentaron mayor efecto bactericida fueron el 2 y el 3 (muestras colombianas) luego de 48 horas de incubación.El mejor efecto bacteriostático lo presentó la muestra 2 (propóleo colombiano) a un periodo de incubación de 24 horas. El 70% de las muestras de propóleo incrementaron su actividad luego de un tiempo de incubación de 48 horas, en relación con el efecto detectado a las 24 horas. A mayor exposición de las bacterias al propóleo, las muestras colombianas mostraron un efecto superior, las argentinas un efecto moderado y las demás muestras (30%), permanecieron estables.
It was evaluated the antimicrobial activity of four propolis extracts from Argentina, five from Colombia and one from Cuba, using Streptococcus mutans ATCC 25175. The samples were kept in a 96% ethylic alcohol solution, remaining a concentration of 3%. The bactericidal and bacteriostatic activity was measured by the minimun inhibitory concentration among a range of 0.02 and 15mg/ml. All of the analyzed samples showed activity against Streptococcus Mutans when they are used to concentrations 15 a 3.75 mg/ml. The propolis that presented the highest effect bactericidal was number 2 and 3 (sample from Colombia) after 48 hours of incubation.The highest effect bacteriostatic was presented by the sample 2 (propolis from Colombia) after 24 hours of incubation. 70% of the propóleo samples increased their activity after a time of incubation of 48 hours compare to the effect shown to the 24 hours. All the Colombian samples showed a superior effect, when the bacteria were exposed to the propóleo for a longer period of time; in two of these cases until of five dilutions, samples 2 and 3, this effect was presented in a moderate way, using the argentine samples 6 and 10 while the other samples (30%) remained steady.
