Acceso usuarios
María Monsalve Moreno
This study evaluated the intestinal effects of alkalinized filtered water in normal or obese adult Zucker rats. 12 weeksold rats consumed for three months either tap water or filtered alkalinized tap water from Madrid city.
Weight gain was monitored, changes in metabolism were evaluated by indirect calorimetry, total antioxidant capacity and levels of inflammatory mediators were measured in plasma. Feces were collected and their microbial composition was analyzed, and histological analysis of the small and large intestine was performed, assessing the general state of the mucosa (MUC2), the inflammatory state (F4/80) and the presence of oxidative modifications in proteins (HNE) by immunofluorescence (IF) and immunohistochemistry (IHC). The results obtained showed that the consumption of alkalinized filtered water improved the composition of the intestinal microbiome and the state of the intestinal mucosa, reduced both local and systemic inflammation and the level of oxidative stress. These changes were accompanied by a better maintenance in the oxidative status of the rats.
No differences were observed in antioxidant capacity nor in weight gain. The incorporation of probiotics in the diet had a significant impact on the microbiome. These effects were indicative of an improvement in general metabolic, oxidative and inflammatory status.
